Index: biology/pooler/Makefile
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--- biology/pooler/Makefile	(nonexistent)
+++ biology/pooler/Makefile	(working copy)
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+# $FreeBSD$
+
+PORTNAME=pooler
+DISTVERSION=1.73
+CATEGORIES=biology
+
+MAINTAINER=ssb22@cam.ac.uk
+COMMENT=PrimerPooler: optimise primer-set combinations
+
+LICENSE=APACHE20
+
+MAKE_FLAGS=-C pooler -f
+USES=gmake
+
+USE_GITHUB=yes
+GH_ACCOUNT=ssb22
+GH_PROJECT=PrimerPooler
+GH_TAGNAME=v1.73
+
+PLIST_FILES=bin/pooler man/man1/pooler.1.gz
+
+.include <bsd.port.mk>

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Index: biology/pooler/distinfo
===================================================================
--- biology/pooler/distinfo	(nonexistent)
+++ biology/pooler/distinfo	(working copy)
@@ -0,0 +1,3 @@
+TIMESTAMP = 1605112657
+SHA256 (ssb22-PrimerPooler-1.73-v1.73_GH0.tar.gz) = 84bd453cc771240294c921b5674911aa94b36b34b7ce5eb37c0829b6d197814c
+SIZE (ssb22-PrimerPooler-1.73-v1.73_GH0.tar.gz) = 125731

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Index: biology/pooler/pkg-descr
===================================================================
--- biology/pooler/pkg-descr	(nonexistent)
+++ biology/pooler/pkg-descr	(working copy)
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+This is a program I wrote for cancer researchers and others who want to use
+Multiplex PCR to study DNA samples, and wish to optimise their combinations of
+primers to minimise the formation of dimers.
+
+Primer Pooler can:
+
+* Check through each proposed pool for combinations that are likely to form
+  dimers,
+
+* Automatically move prospective amplicons between proposed pools to reduce
+  dimer formation,
+
+* Automatically search the genome sequence to find which amplicons overlap, and
+  place their corresponding primers in separate pools,
+
+* Optionally keep pool sizes within a specified range,
+
+* Handle thousands of primers without being slow (useful for high-throughput
+  sequencing applications),
+
+* Do all of the above with degenerate primers too.
+
+If your CPU is modern enough to have them, Primer Pooler will take advantage of
+64-bit registers and multiple cores. But it also runs on older equipment.
+
+WWW: http://ssb22.user.srcf.net/pooler/
+

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