diff --git a/biology/sra-tools/Makefile b/biology/sra-tools/Makefile
new file mode 100644
index 000000000000..4cbe2c298357
--- /dev/null
+++ b/biology/sra-tools/Makefile
@@ -0,0 +1,90 @@
+# $FreeBSD$
+
+PORTNAME=	sra-tools
+DISTVERSION=	2.11.0
+CATEGORIES=	biology
+
+MAINTAINER=	mzaki@niid.go.jp
+COMMENT=	NCBI's toolkit for handling data in INSDC Sequence Read Archives
+
+LICENSE=	PD LGPL21+
+LICENSE_COMB=	multi
+LICENSE_FILE_PD=	${WRKSRC}/LICENSE
+LICENSE_DISTFILES_LGPL21+ =
+
+BUILD_DEPENDS=	bash:shells/bash \
+		${LOCALBASE}/lib/libngs-c++.a:biology/ngs-sdk \
+		${NONEXISTENT}:biology/ncbi-vdb:build
+LIB_DEPENDS=	libxml2.so:textproc/libxml2 \
+		libhdf5.so:science/hdf5 \
+		libmbedtls.so:security/mbedtls
+
+USE_GITHUB=	yes
+GH_ACCOUNT=	ncbi
+
+USES=		gmake perl5 shebangfix
+USE_PERL5=	build
+SHEBANG_GLOB=	*.sh *.pl
+
+GNU_CONFIGURE=	yes
+
+NCBI_VDB_WRKSRC=	$$(make -C ${PORTSDIR}/biology/ncbi-vdb -V WRKSRC)
+NCBI_VDB_FILESDIR=	$$(make -C ${PORTSDIR}/biology/ncbi-vdb -V FILESDIR)
+
+CONFIGURE_ARGS=	--with-ngs-sdk-prefix=${LOCALBASE} \
+		--with-ncbi-vdb-sources=${NCBI_VDB_WRKSRC} \
+		--with-xml2-prefix=${LOCALBASE} \
+		--with-hdf5-prefix=${LOCALBASE} \
+		CC="${CC}" CXX="${CXX}" TOOLS="${CHOSEN_COMPILER_TYPE}" \
+		--with-ncbi-vdb-build=${NCBI_VDB_WRKSRC}/amd64-portbld-freebsd12.2
+#		-d
+#		--with-mbedtls-prefix=${LOCALBASE}
+MAKE_ARGS=	CCNAME=${CC} CXXNAME=${CXX} PKGCFLAGS="${CFLAGS}" \
+		INST_ETCDIR=${STAGEDIR}${PREFIX}/etc
+
+ONLY_FOR_ARCHS=	amd64 i386
+ONLY_FOR_ARCHS_REASON=	NCBI-VDB requires SSE2 instructions
+BROKEN_i386=	buggy output
+
+CFLAGS+=	-I${WRKSRC}/interfaces/os/sun
+CXXFLAGS+=	-I${WRKSRC}/interfaces/os/sun
+LDFLAGS+=	-lz
+
+BINARY_ALIAS=	gcc=${CC} g++=${CXX}
+
+# for port developers, building with these options may fail
+OPTIONS_DEFINE=	DEBUG OPTIMIZED_CFLAGS TEST
+DEBUG_PREVENTS=	OPTIMIZED_CFLAGS
+DEBUG_CONFIGURE_WITH=	debug
+OPTIMIZED_CFLAGS_MAKE_ARGS_OFF=	OPT=""
+TEST_TEST_TARGET=	test
+TEST_USES=	python
+
+xpre-patch:
+	@${CP} ${FILESDIR}/Makefile.bsd ${WRKSRC}/build/
+	@for f in ${WRKSRC}/build/ld.linux.*.sh; do ${CP} -p $${f} $${f%linux*}bsd$${f#*linux}; done
+	@${CP} -p ${WRKSRC}/build/ld.linux.gcc.sh ${WRKSRC}/build/ld.bsd.clang.sh
+
+pre-configure:
+	${CP} ${NCBI_VDB_WRKSRC}/setup/os-arch.prl ${WRKSRC}/setup/os-arch.prl
+	# same as in biology/ncbi-vdb
+	@${CP} ${NCBI_VDB_FILESDIR}/Makefile.bsd ${WRKSRC}/build/
+	@${CP} ${NCBI_VDB_WRKSRC}/build/ld.bsd.*.sh ${WRKSRC}/build/
+	${MKDIR} ${WRKSRC}/interfaces/override
+	@${CP} ${NCBI_VDB_WRKSRC}/interfaces/os/sun/atomic32.h ${WRKSRC}/interfaces/override
+	@${CP} ${NCBI_VDB_WRKSRC}/interfaces/os/sun/byteswap.h ${WRKSRC}/interfaces/override
+	@for f in ${NCBI_VDB_WRKSRC}/build/ld.linux.*.sh; do ${CP} -p $${f} $${f%linux*}bsd$${f#*linux}; done
+	@${CP} -p ${NCBI_VDB_WRKSRC}/build/ld.linux.gcc.sh ${WRKSRC}/build/ld.bsd.clang.sh
+	#@${MKDIR} ${WRKSRC}/interfaces/os/bsd
+	#@${CP} -p ${WRKSRC}/interfaces/os/mac/endian.h ${WRKSRC}/interfaces/os/bsd/
+	${LN} -s ${NCBI_VDB_WRKSRC}/interfaces ${WRKSRC}/interfaces
+
+post-patch-TEST-on:
+	${REINPLACE_CMD} -e 's#python#${PYTHON_CMD}#' ${WRKSRC}/test/kget/Makefile
+
+xpost-configure:
+	@${ECHO} ${CHOSEN_COMPILER_TYPE} > ${WRKSRC}/build/COMP
+
+post-build-TEST-on:	do-test
+
+.include <bsd.port.mk>
diff --git a/biology/sra-tools/distinfo b/biology/sra-tools/distinfo
new file mode 100644
index 000000000000..98c75c320f18
--- /dev/null
+++ b/biology/sra-tools/distinfo
@@ -0,0 +1,3 @@
+TIMESTAMP = 1617221199
+SHA256 (ncbi-sra-tools-2.11.0_GH0.tar.gz) = 10ac0a4d1fafc274bc107de811891d3e803d0713a247581dece4448231883810
+SIZE (ncbi-sra-tools-2.11.0_GH0.tar.gz) = 8966605
diff --git a/biology/sra-tools/files/patch-setup_install.perl b/biology/sra-tools/files/patch-setup_install.perl
new file mode 100644
index 000000000000..f1600db36d19
--- /dev/null
+++ b/biology/sra-tools/files/patch-setup_install.perl
@@ -0,0 +1,13 @@
+--- setup/install.perl.orig	2021-03-31 20:29:55 UTC
++++ setup/install.perl
+@@ -1283,8 +1283,8 @@ sub prepare {
+             '   $_{INST_JARDIR  } = "$_{PREFIX}/jar"; ' .
+             '   $_{INST_SHAREDIR} = "$_{PREFIX}/share"; ' .
+             '   $_{INCDIR       } = "$Bin/../include"; ' .
+-            '   $_{LIBDIR64     } = "$Bin/../lib64"; ' .
+-            '   $_{LIBDIR32     } = "$Bin/../lib32"; ';
++            '   $_{LIBDIR64     } = "$Bin/../lib"; ' .
++            '   $_{LIBDIR32     } = "$Bin/../lib"; ';
+         if ($HAVE{TWO_LIBS}) {
+             $code .=
+                '$_{INST_NGSLIBDIR} = "' . "$root/usr/local/ngs/ngs-sdk/lib\";"
diff --git a/biology/sra-tools/files/patch-setup_konfigure.perl b/biology/sra-tools/files/patch-setup_konfigure.perl
new file mode 100644
index 000000000000..aacfe898be6d
--- /dev/null
+++ b/biology/sra-tools/files/patch-setup_konfigure.perl
@@ -0,0 +1,121 @@
+--- setup/konfigure.perl.orig	2021-03-15 18:25:25 UTC
++++ setup/konfigure.perl
+@@ -204,7 +204,7 @@ print "checking system type... " unless ($AUTORUN);
+ my ($OS, $ARCH, $OSTYPE, $MARCH, @ARCHITECTURES) = OsArch();
+ println $OSTYPE unless ($AUTORUN);
+ 
+-unless ($OSTYPE =~ /linux/i || $OSTYPE =~ /darwin/i || $OSTYPE eq 'win') {
++unless ($OSTYPE =~ /linux/i || $OSTYPE =~ /darwin/i || $OSTYPE eq 'win' || $OSTYPE =~ /freebsd/i) {
+     println "configure: error: unsupported system '$OSTYPE'";
+     exit 1;
+ }
+@@ -225,7 +225,7 @@ if ($OS eq 'linux') {
+ 
+ print "checking machine architecture... " unless ($AUTORUN);
+ println $MARCH unless ($AUTORUN);
+-unless ($MARCH =~ /x86_64/i || $MARCH =~ /i?86/i || $MARCH =~ /aarch64/) {
++unless ($MARCH =~ /x86_64/i || $MARCH =~ /amd64/i || $MARCH =~ /i?86/i || $MARCH =~ /aarch64/) {
+     println "configure: error: unsupported architecture '$OSTYPE':'$MARCH'";
+     exit 1;
+ }
+@@ -310,7 +310,7 @@ print "checking for supported architecture... " unless
+ 
+ my $BITS;
+ 
+-if ($MARCH =~ /x86_64/i) {
++if ($MARCH =~ /x86_64/i || $MARCH =~ /amd64/i) {
+     $BITS = 64;
+ } elsif ($MARCH eq 'fat86') {
+     $BITS = '32_64';
+@@ -337,6 +337,16 @@ if ($OSTYPE =~ /linux/i) {
+     $OSINC = 'unix';
+     $TOOLS = 'gcc' unless ($TOOLS);
+     $PYTHON = 'python';
++} elsif ($OSTYPE =~ /freebsd/i) {
++    $LPFX = 'lib';
++    $OBJX = 'o';
++    $LOBX = 'pic.o';
++    $LIBX = 'a';
++    $SHLX = 'so';
++    $EXEX = '';
++    $OSINC = 'unix';
++    $TOOLS = 'clang' unless ($TOOLS);
++    $PYTHON = 'python';
+ } elsif ($OSTYPE =~ /darwin/i) {
+     $LPFX = 'lib';
+     $OBJX = 'o';
+@@ -512,7 +522,7 @@ foreach my $href (DEPENDS()) {
+             $I = $t if (-e $t);
+         }
+         push ( @L, File::Spec->catdir($OPT{$o}, 'lib') );
+-        push ( @L, File::Spec->catdir($OPT{$o}, 'lib64') );
++        #push ( @L, File::Spec->catdir($OPT{$o}, 'lib64') );
+     }
+     my ($i, $l) = find_lib($_, $I, @L);
+     if (defined $i || $l) {
+@@ -934,7 +944,7 @@ EndText
+     if ($PKG{LNG} eq 'C') {
+         if ($TOOLS =~ /clang/i) {
+    L($F, 'SONAME  = -install_name ' .
+-               '$(INST_LIBDIR)$(BITS)/$(subst $(VERSION),$(MAJVERS),$(@F)) \\');
++               '$(INST_LIBDIR)/$(subst $(VERSION),$(MAJVERS),$(@F)) \\');
+    L($F, '    -compatibility_version $(MAJMIN) -current_version $(VERSION) \\');
+    L($F, '    -flat_namespace -undefined suppress');
+         } else {
+@@ -1043,7 +1053,7 @@ EndText
+     L($F, '# directory rules');
+     if ($PKG{LNG} eq 'C') {
+         L($F, '$(BINDIR) $(LIBDIR) $(ILIBDIR) '
+-            . '$(OBJDIR) $(INST_LIBDIR) $(INST_LIBDIR)$(BITS):');
++            . '$(OBJDIR) $(INST_LIBDIR) $(INST_LIBDIR):');
+         T($F, 'mkdir -p $@');
+     } elsif ($PKG{LNG} eq 'JAVA') {
+         # test if we have jni header path
+@@ -1076,7 +1086,7 @@ EndText
+     if ($OS eq 'linux' || $OS eq 'mac') {
+         L($F, '# installation rules');
+         L($F,
+-        '$(INST_LIBDIR)$(BITS)/%.$(VERSION_LIBX): $(LIBDIR)/%.$(VERSION_LIBX)');
++        '$(INST_LIBDIR)/%.$(VERSION_LIBX): $(LIBDIR)/%.$(VERSION_LIBX)');
+         T($F, '@ echo -n "installing \'$(@F)\'... "');
+         T($F, '@ if cp $^ $@ && chmod 644 $@;                         \\');
+         T($F, '  then                                                 \\');
+@@ -1087,7 +1097,7 @@ EndText
+         T($F, '      ln -s $(patsubst %$(VERSION),%$(MAJVERS),$(@F)) '
+                       . '$(patsubst %$(VERSION_LIBX),%$(LIBX),$@); \\');
+         T($F, '      ln -s $(patsubst %$(VERSION_LIBX),%$(LIBX),$(@F)) ' .
+-   '$(INST_LIBDIR)$(BITS)/$(patsubst %.$(VERSION_LIBX),%-static.$(LIBX),$(@F));'
++   '$(INST_LIBDIR)/$(patsubst %.$(VERSION_LIBX),%-static.$(LIBX),$(@F));'
+                                                               . ' \\');
+         T($F, '      echo success;                                    \\');
+         T($F, '  else                                                 \\');
+@@ -1097,7 +1107,7 @@ EndText
+         L($F);
+ 
+         L($F,
+-        '$(INST_LIBDIR)$(BITS)/%.$(VERSION_SHLX): $(LIBDIR)/%.$(VERSION_SHLX)');
++        '$(INST_LIBDIR)/%.$(VERSION_SHLX): $(LIBDIR)/%.$(VERSION_SHLX)');
+         T($F, '@ echo -n "installing \'$(@F)\'... "');
+         T($F, '@ if cp $^ $@ && chmod 755 $@;                         \\');
+         T($F, '  then                                                 \\');
+@@ -1168,9 +1178,9 @@ EndText
+ 	    print $F "    \$_{INCDIR       } = '" . expand("$Bin/.."      ) . "';\n";
+ 	    if ($PKG{LNG} ne 'PYTHON') {
+ 	        print $F "  \$_{BINDIR$BITS} = '" . expand($E_BINDIR      ) . "';\n";
+-	        print $F "  \$_{LIBDIR$BITS} = '" . expand($E_LIBDIR      ) . "';\n";
++	        print $F "  \$_{LIBDIR} = '" . expand($E_LIBDIR      ) . "';\n";
+ 	    } elsif ($OPT{PYTHON_LIB_PATH}) {
+-	        print $F "  \$_{LIBDIR$BITS} = '$OPT{PYTHON_LIB_PATH}';\n";
++	        print $F "  \$_{LIBDIR} = '$OPT{PYTHON_LIB_PATH}';\n";
+ 	    }
+ 	    print $F "    \$_{OTHER_PREFIX } = '$PKG{UPATH}';\n";
+ 	    print $F "    \$_{PREFIX       } = '$OPT{'prefix'}';\n";
+@@ -1406,7 +1416,7 @@ sub find_in_dir {
+                 ++$found;
+             }
+             if (! $found) {
+-                my $libdir = File::Spec->catdir($dir, 'lib' . $BITS);
++                my $libdir = File::Spec->catdir($dir, 'lib');
+                 my $f = File::Spec->catdir($libdir, $lib);
+                 print "\tchecking $f\n\t" if ($OPT{'debug'});
+                 if (-e $f) {
diff --git a/biology/sra-tools/files/patch-tools_driver-tool_cmdline.cpp b/biology/sra-tools/files/patch-tools_driver-tool_cmdline.cpp
new file mode 100644
index 000000000000..46747e4840e5
--- /dev/null
+++ b/biology/sra-tools/files/patch-tools_driver-tool_cmdline.cpp
@@ -0,0 +1,13 @@
+--- tools/driver-tool/cmdline.cpp.orig	2021-04-21 19:42:12 UTC
++++ tools/driver-tool/cmdline.cpp
+@@ -50,6 +50,10 @@
+ #define environ (*_NSGetEnviron())
+ #endif
+ 
++#if defined(__FreeBSD__)
++extern char **environ;
++#endif
++
+ #define TRACE( lvl, ... ) /* ignore */
+ 
+ namespace ncbi
diff --git a/biology/sra-tools/pkg-descr b/biology/sra-tools/pkg-descr
new file mode 100644
index 000000000000..2e56f949b113
--- /dev/null
+++ b/biology/sra-tools/pkg-descr
@@ -0,0 +1,36 @@
+SRA tools is a toolkit for using data in the INSDC Sequence Read Archives.
+
+SRAs operated by International Nucleotide Sequence Database Collaboration
+houses sequence reads and alignments generated by "next-gen" sequencers.
+SRA tools allows conversion of .sra files, which INSDC SRAs maintain,
+from/to other formats that the 'next-gen' sequenecers generate including:
+
+* csfasta/csqual (ABI SOLiD)
+* fastq (and fasta for writing)
+* hdf5 (PacBio, reading only)
+* qseq (older Illumina)
+* sam (writing only) / bam (reading only)
+* sff
+
+The toolkit uses NCBI-VDB back-end enabling seamless access to remote SRA data
+and local SRA files.
+
+WWW: https://github.com/ncbi/sra-tools/wiki
+SRA tools is a toolkit for using data in the INSDC Sequence Read Archives.
+
+SRAs operated by International Nucleotide Sequence Database Collaboration
+houses sequence reads and alignments generated by "next-gen" sequencers.
+SRA tools allows conversion of .sra files, which INSDC SRAs maintain,
+from/to other formats that the 'next-gen' sequenecers generate including:
+
+* csfasta/csqual (ABI SOLiD)
+* fastq (and fasta for writing)
+* hdf5 (PacBio, reading only)
+* qseq (older Illumina)
+* sam (writing only) / bam (reading only)
+* sff
+
+The toolkit uses NCBI-VDB back-end enabling seamless access to remote SRA data
+and local SRA files.
+
+WWW: https://github.com/ncbi/sra-tools/wiki
diff --git a/biology/sra-tools/pkg-plist b/biology/sra-tools/pkg-plist
new file mode 100644
index 000000000000..ea8e06df3cf0
--- /dev/null
+++ b/biology/sra-tools/pkg-plist
@@ -0,0 +1,82 @@
+bin/abi-dump
+bin/abi-load
+bin/align-info
+bin/bam-load
+bin/cache-mgr
+bin/cg-load
+bin/copycat
+bin/fastdump
+bin/fastq-dump
+bin/fastq-load
+bin/helicos-load
+bin/illumina-dump
+bin/illumina-load
+bin/kar
+bin/kdbmeta
+bin/latf-load
+bin/pacbio-load
+bin/prefetch
+bin/rcexplain
+bin/sam-dump
+bin/sff-dump
+bin/sff-load
+bin/sra-pileup
+bin/sra-sort
+bin/sra-stat
+bin/srapath
+bin/srf-load
+bin/test-sra
+bin/vdb-config
+bin/vdb-copy
+bin/vdb-decrypt
+bin/vdb-dump
+bin/vdb-encrypt
+bin/vdb-get
+bin/vdb-lock
+bin/vdb-passwd
+bin/vdb-unlock
+bin/vdb-validate
+etc/ncbi/certs.kfg
+etc/ncbi/default.kfg
+etc/ncbi/vdb-copy.kfg
+bin/abi-dump
+bin/abi-load
+bin/align-info
+bin/bam-load
+bin/cache-mgr
+bin/cg-load
+bin/copycat
+bin/fastdump
+bin/fastq-dump
+bin/fastq-load
+bin/helicos-load
+bin/illumina-dump
+bin/illumina-load
+bin/kar
+bin/kdbmeta
+bin/latf-load
+bin/pacbio-load
+bin/prefetch
+bin/rcexplain
+bin/sam-dump
+bin/sff-dump
+bin/sff-load
+bin/sra-pileup
+bin/sra-sort
+bin/sra-stat
+bin/srapath
+bin/srf-load
+bin/test-sra
+bin/vdb-config
+bin/vdb-copy
+bin/vdb-decrypt
+bin/vdb-dump
+bin/vdb-encrypt
+bin/vdb-get
+bin/vdb-lock
+bin/vdb-passwd
+bin/vdb-unlock
+bin/vdb-validate
+etc/ncbi/certs.kfg
+etc/ncbi/default.kfg
+etc/ncbi/vdb-copy.kfg